hcr hybridization buffer  (Molecular Instruments)

Journal: STAR Protocols

Article Title: Protocol for multiplex whole-mount RNA fluorescence in situ hybridization combined with immunohistochemistry in the mosquito brain

doi: 10.1016/j.xpro.2025.104109

Figure Lengend Snippet: Example of multiplex whole-mount RNA fluorescent HCR in situ hybridization combined with immunohistochemistry in the mosquito brain (A) Whole mosquito brain showing RNA fluorescent HCR in situ hybridization against putative GAD1 ( AGAP005866 ) and SerT ( AGAP010621 ) and immunohistochemistry against PDF and DAPI. (B–B″″) Maximum projection (∼2 μm total) of the optic lobe showing non-overlapping cell bodies of GAD1 + and SerT + cells and PDF neurites. Individual panels show fluorescence channels imaged using the following excitation wavelengths: 488 nm (PDF), 546 nm ( GAD1 ), 647 nm ( SerT ), and 358 nm (DAPI). See also and .

Article Snippet: HCR probe hybridization buffer , Molecular Instruments , N/A.

Techniques: Multiplex Assay, In Situ Hybridization, Immunohistochemistry, Fluorescence

Journal: Developmental biology

Article Title: PRDM paralogs are required for Meckel’s cartilage formation during mandibular bone development

doi: 10.1016/j.ydbio.2025.12.002

Figure Lengend Snippet: (A,B-A””,B””) Hybridization chain reaction (HCR) was performed on coronal sections of E10.5 mandibular processes to assess gene expression patterns for Dlx5 (green) (A-B), Dlx6 (cyan) (A’-B’), Hand2 (magenta) (A”-B”), Sox10 (yellow) (A”’-B”’) in controls and double mutants. (A””-B””) Merged image of A-A”’ and B-B”’ with the counterstain DAPI in blue. (C) RT-qPCR for the indicated mRNA transcripts isolated from the mandibular processes of control and Prdm3;Prdm16 double mutants at E10.5 (n = 4 for both control and mutants). (D) RT-qPCR for Sox10 at E10.5 (n = 4 for both control and mutants) and E11.5 (n = 3 for control, n = 4 for mutants). (E-F, E’-F’) HCR was performed on coronal sections of E11.5 mandibular processes to assess gene expression patterns for Sox10 . (E’-F’) Merged image of E and F with the counterstain DAPI in blue. Double white arrowhead denotes the initial presumptive cranial ganglia and peripheral nerve tracks entering the mandibular prominence in control animals. White arrow indicates areas of Sox10 expression that are expanded in the mesenchyme in the Prdm3;Prdm16 double mutants. Abbreviations: MdP, mandibular process. *P≤0.05; (unpaired, two-tailed Student’s t test).

Article Snippet: Sections were pre-hybridized in HCR HiFi Probe Hybridization Buffer (Molecular Instruments) for 10 minutes at 37°C in a humidified chamber.

Techniques: Expressing, Hybridization, Gene Expression, Quantitative RT-PCR, Isolation, Control, Two Tailed Test